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小鼠成纖維細胞
- 品牌：北納生物
- 型號： BNCC341885
- 產地：北京 朝陽區
- 供應商報價：面議
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北京北納創聯生物技術研究院
更新時間：2022-07-22 10:46:09 -
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詳細介紹
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資源編號：BNCC341885
英文名： McCoy
類型： 貼壁
培養方法
培養基 Complete growth medium: 1640，90%；FBS，10%；雙抗。 傳代方法 1.Remove and discard culture medium. 2.Briefly rinse the cell layer with 3-5ml PBS solution to remove all traces of serum that contains trypsin inhibitor. 3.Add 1.0 ml of 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed. Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. 4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. 5.Add appropriate aliquots of the cell suspension to new culture vessels. Removal of Trypsin by centrifugation is recommended. 6.Incubate cultures at 37°C. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium renewal: 2 times per week 生長條件 Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% 存儲條件 Freeze medium: FBS/NBS, 92%; DMSO, 8%（for reference） Storage temperature: liquid nitrogen vapor phase
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