質粒類型：哺乳動物表達載體

啟動子：CMV

表達水平：高

克隆方法：多克隆位點，限制性內切酶

載體大小：3114 bp 

5' 測序引物：CMV-F

5' 測序引物序列:5'-CGCAAATGGGCGGTAGGCGTG-3'

載體抗性：Ampicillin (氨芐青霉素)

pBI-CMV1 is a mammalian bidirectional expression vector that allows the constitutive expression of two proteins of interest. Protein expression is driven by one of two constitutively active, minimal human cytomegalovirus promoters, PminCMV1 and PminCMV2, located just upstream of two independent multiple cloning sites (MCS 1 and MCS 2, respectively). To allow propagation and selection in E. coli, the vector also contains a ColE1 origin of replication and an ampicillin resistance gene (Ampr). 

Use: pBI-CMV1 is designed to simultaneously and constitutively express two genes of interest. Each gene must be cloned into a different MCS, and contain both an initiation codon and a stop codon. The pBI-CMV1 vector can be transfected into mammalian cells using any standard transfection method.