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Recombinant Human GIP Protein,hFc Tag

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詳細介紹

性能參數

表達區間及表達系統(Source

Human GIP Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Glu22-Gln93.[Accession | P09681]

分子量大小(Molecular Weight

The protein has a predicted MW of 34.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

內毒素(Endotoxin

Less than 1EU per μg by the LAL method.

純度(Purity

> 95% as determined by Tris-Bis PAGE and HPLC

活性(Activity

ELISA Data: Immobilized Human GIP, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-GIP Antibody, hFc Tag with the EC50 of 88.1ng/ml determined by ELISA. 

制劑(Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重構方法(Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

儲存條件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~8 °C under sterile conditions after reconstitution.

3 months, -25~-15℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事項

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

 

Ver. CN20230625

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