分子別名（Synonyms）

VSTM3; VSIG9;TIGIT;

表達區間及表達系統（Source）

Biotinylated Human TIGIT Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Met22-Pro141.[Accession | Q495A1]

分子量大?。∕olecular Weight）

The protein has a predicted MW of 16.1 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on SDS-PAGE result.

內毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

純度（Purity）

> 95% as determined by SDS-PAGE and HPLC.

活性（Activity）

ELISA Data: Immobilized Biotinylated Human TIGIT, His-Avi Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human CD155, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA.

制劑（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重構方法（Reconstitution）

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.