表達區間及表達系統（Source）

Human RGMa Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Cys48-Gly422.[Accession | Q96B86-1]

分子量大小（Molecular Weight）

The protein has a predicted MW of 44.5 kDa. Due to glycosylation, the protein migrates to 45-48 kDa based on Tris-Bis PAGE result.

內毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

純度（Purity）

> 95% as determined by Tris-Bis PAGE and HPLC

活性（Activity）

ELISA Data: Immobilized Human RGMa, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human Neogenin, hFc Tag with the EC50 of 59.2ng/ml determined by ELISA. 

制劑（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

重構方法（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.