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Recombinant Cynomolgus GFRAL/GFR alpha-like Protein,His-Avi Tag

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詳細介紹

 

性能參數(shù)

分子別名(Synonyms)

GRAL; GFRAL;GFR alpha-like; GFR alpha like

表達區(qū)間及表達系統(tǒng)(Source)

Cynomolgus GFRAL/GFR alpha-like Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus. It contains Gln20-Glu351.[Accession | XP_015304775.2]

分子量大小(Molecular Weight)

The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on SDS-PAGE result.

內(nèi)毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

純度(Purity)

> 95% as determined by SDS-PAGE and HPLC.

活性(Activity)

ELISA Data: Immobilized Cynomolgus GFRAL, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Cynomolgus GDF15, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA.

制劑(Formulation)

Lyophilized from 0.22μm filtered solution in 20mM PB, 0.4M NaCl (pH 6.0). Normally 8% trehalose is added as protectant before lyophilization.

重構(gòu)方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 20mM PB, 0.4M NaCl (pH 6.0).

 

儲存條件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事項

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

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