分子別名（Synonyms）

PD-L1; PDL1;CD274;B7-H

表達區(qū)間及表達系統(tǒng)（Source）

Human PD-L1/B7-H1 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus. It contains Phe19-Arg238.[Accession | Q9NZQ7-1]

分子量大小（Molecular Weight）

The protein has a predicted MW of 50.9 kDa. Due to glycosylation, the protein migrates to 68-75 kDa based on SDS-PAGE result.

內(nèi)毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

純度（Purity）

> 95% as determined by SDS-PAGE and HPLC.

活性（Activity）

ELISA Data: Immobilized Human PD-L1, mFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-PD-L1 Antibody, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA .Immobilized Human PD-L1, mFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human PD-1, hFc Tag with the EC50 of 0.13μg/ml determined by ELISA.

制劑（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重構(gòu)方法（Reconstitution）

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.